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Coding

Part:BBa_K1122007:Design

Designed by: Lina Gasiūnaitė   Group: iGEM13_Edinburgh   (2013-08-13)

AmilCP (BBa_K592009) compatible with Genabler assembly


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

To be compatible with Genabler assembly, the part has inward-facing EarI sites at the ends. When assembled by BioBrick assembly, five N-terminal residues MetAlaSerSerSer- and one C-terminal Ala residue will be added to the original sequence of BBa_K592009. When assembled with normal BioBrick assembly, the part contains a start and a stop codon, however, if Genabler assembly is used, the resulting part does not contain either of the codons. Such design enables to use standardised linkers that contain start and stop codons and variable upstream sequence in Genabler assembly.

Source

AmilCP is based on the BioBrick BBa_K592009.

References

Alieva, N. O., et al. 2008. Diversity and evolution of coral fluorescent proteins. PLoS One 3:e2680.